Gene therapy in Orthopaedics - DNB Orthopaedics MS Orthopedics MRCS Exam GUIDE - Orthodnb.com

DNB Orthopaedics  MS Orthopedics  MRCS Exam GUIDE - Orthodnb.com

Orthopaedic Guidance for DNB Orthopaedics,MS Orthopaedics and Mrcs exams.Cme courses for orthopaedic surgeons.Davangere notes,solved question papers.DNB Ortho,MS Ortho MRCS,Exam Guide orthodnb.com videos and notes 9087747888 orthoguidance@gmail.com

Breaking

Home Top Ad

Post Top Ad

Tuesday 16 January 2018

Gene therapy in Orthopaedics

GENE THERAPY in orthopaedics


  • Definition
    • treatment directed to cure genetic diseases by introducing normal genes into patients to overcome the effect of defective genes
    • these genes delivers individual proteins to specific cells
  • History
    • I therapeutic trial : 1990 for SCID in ADA deficiency
    • Orthopaedics : only in RA
  • Design
    • includes
      • gene for specific protein (transgene)
      • identify target tissue
      • choosing vector and vector delivery method
      • choosing a promoter to regulate gene
Get Davanagere Recent advances volume for recent advances in orthopaedics for MS DNB Ortho exams Click Here

    • Somatic gene therapy : targets non-reproductive cells
    • Germ line gene therapy : targets gamete cells – manipulations are passed to progeny
    • viral vectors
      • done by viral transduction
      • genetically engineered virus permitted to initially infect the cell – further replication inside cell is prevented
      • can not infect other cells d/t deficiency in replication
      • ADV, AAV, HSV, Retrovirus
    • non viral vectors
      • done by transfection (eukaryotic cells made to take up foreign DNA from environment)
      • not as effective as viral vectors
      • Naked DNA
      • DNA -protein complex
      • Liposomes ( for delivering genes to chondrocytes)
      • Gene gun ( for muscles and cartilage cells)
    • Systemic gene therapy
      • targets all cells
      • injected directly to blood
      • treating metastatic diseases
      • poor penetration in tissues with decreased blood supply – cartilage and menisci
    • Local gene therapy
      • injected to specific tissue
      • direct
        • ACL, MCL, Meniscus, Tendon, Ligament
      • indirect
        • target cell is removed form body – exposed to vector in vitro and reinserted
        • spine, articular cartilage, skeletal muscle
  • Scope in Orthopaedics
    • Rheumatoid arthritis
      • indirect gene therapy
      • synovial cells harvested – transduced to produce IRAP – injected back to diseased joint
      • IRAP blocks IL-1 intraarticularly
    • Osteochondral defect
      • indirect delivery of genes encoding BMP-2, IGF-1, TGF-beta to articular cartilage – increases proteoglycan synthesis
    • Tendons and ligaments
      • better integration in ligaments injury reconstruction if engineered to express BMP-2, IGF, PDGF
    • Intervertebral disc
      • direct delivery of TGF-beta to nucleus pulposus – increased proteoglycan synthesis
    • Skeletal muscle
      • indirect gene therapy
      • basal lamina of muscles can not be penetrated by virus
      • IGF-1 and NGF improve muscle healing
    • Spine fusion
      • BMP-2 injections : solid fusion
    • Bone healing
      • direct
        • using adeno virus with BMP gene
        • immune response to virus can occur
      • indirect
        • with osteogenic precursors and stem cells
  • definitive clinical trials only done for Rheumatoid arthritis
  • more research going on
Order Books Videos Notes and study material shop.orthodnb.com

Orthopaedics made simple for DNB MS MRCS Support and Guidance for DNB Orthopaedics, MS Orthopaedics and Orthopaedic Surgeons. DNB Ortho MS Ortho MRCS Exam Guide Diplomate of National Board.Our site has been helping dnb ortho post graduates since a long time.It has been providing the dnb ortho theory question papers,dnb orthopedics solved question bank, davangere orthopaedic notes, sion orthopedic notes.We provide guidance to post graduates as to how to pass dnb and ms ortho exams, and aspiring orthopaedic surgeons surgical technique teaching videos and orthopaedic books and pdf.
Get updates email orthoguidance@gmail.com whatsapp 9087747888

12 comments:

  1. Women somatic therapist You made such an interesting piece to read, giving every subject enlightenment for us to gain knowledge. Thanks for sharing the such information with us to read this...

    ReplyDelete
  2. Somatic coherence techniques Thank you because you have been willing to share information with us. we will always appreciate all you have done here because I know you are very concerned with our.

    ReplyDelete
  3. Numerous guides are not prepared in this training and to improve customer movement maybe it should be important for the helpful preparing model. https://masakor.com/include/category_view2.php?category1=20191203164840_7578

    ReplyDelete
  4. The Museum of Modern Art in New York has a program called "Meet and MOMA.reiki near me

    ReplyDelete
  5. enterprising self again and most importantly finally she came to realize that menopause meant only the end of reproductive life of a woman; it is not the end of life. bioidentical hormone therapy dallas

    ReplyDelete
  6. When you get a referral from someone you know, they often will tell you their impression or opinion about the therapist they are recommending, and that is a valuable piece of information you won't get if you use online sources. therapist

    ReplyDelete
  7. Let's look at an example. An individual is so rich that he owns large areas of real estate in Florida. To add to his luxury he has his own charter fleet of helicopters. Therapist Wayne PA 19087

    ReplyDelete
  8. This is my first visit to your web journal! We are a group of volunteers and new activities in the same specialty. Website gave us helpful data to work. Curso de biomagnetismo

    ReplyDelete
  9. These social interactions are great for your mental health, forcing you to exchange information and ideas with another person. depression therapy

    ReplyDelete
  10. Set aside time for exercise, just like you would for any other important appointment. Weight Loss Service

    ReplyDelete
  11. For the clinical management of orthopaedic disorders that are challenging to treat with conventional surgical or medicinal procedures, gene therapy provides new opportunities. Changes to the signaling peptides involved may be able to treat or alleviate a variety of disorders, including impaired bone healing, the need for significant bone production, cartilage repair, and metabolic bone illnesses. Gene therapy may induce tumor necrosis and boost tumor sensitivity to treatment in orthopaedic oncology. Gene transfer may help heal articular cartilage, menisci, intervertebral disks, ligaments, and tendons, according to growing body of research. These advancements have the potential to revolutionize a variety of musculoskeletal care fields by enabling therapies that are less intrusive, more potent, and more affordable than currently used methods.

    therapy parramatta

    ReplyDelete

Post Bottom Ad

Pages